CST, 99898SF, Whitlow/218 Linker (F2G3S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying . Validated for WB,IHC,F,F. Highly specific and rigorously validated in-house, Whitlow/218 Linker (F2G3S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #99898) is ready to ship. Product Usage Information This product is the carrier free version of product #47414. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 47414 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Whitlow/218 Linker (F2G3S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker. This antibody cross-reacts with a protein of unknown identity at approximately 35 kDa in Jurkat cell extracts. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide. Background The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 55 Source/Isotype: Rabbit IgG