CST, 99913S, mCherry (E5D8F) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying mCherry bacteria. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, mCherry (E5D8F) Mouse Chimeric Monoclonal Antibody (CST #99913) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity mCherry (E5D8F) Mouse Chimeric Monoclonal Antibody detects mCherry-tagged proteins (either N-terminal tagged or C-terminal tagged) exogenously expressed in cells. Please note that the mCherry tags add approximately 28kDa to the molecular weight of the fusion protein. Species Reactivity: All Species Expected Source / Purification This recombinant chimeric antibody is engineered from mCherry (E5D8F) Rabbit mAb #43590 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, mCherry (E5D8F) Rabbit mAb #43590, is produced by immunizing animals with a full-length recombinant mCherry protein. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. mCherry is a monomeric, fluorescent, photostable red dye that was isolated from and further engineered from the Discosoma sea anemone (1). Its peak fluorescent excitation is at 587 nm and emission 610 nm (1). Alternate Names mCherry; MCherry fluorescent protein Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse chimeric IgG2a