CST, 99929S, Synaptophysin (D8F6H) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying SYP. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Synaptophysin (D8F6H) Mouse Chimeric Monoclonal Antibody (CST #99929) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Synaptophysin (D8F6H) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total Synaptophysin protein. Species Reactivity: Human, Mouse, Rat Source / Purification This recombinant chimeric antibody is engineered from Synaptophysin (D8F6H) XP Rabbit mAb #36406 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Synaptophysin (D8F6H) XP Rabbit mAb #36406, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly299 of human Synaptophysin protein. Background Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4). Alternate Names Major synaptic vesicle protein p38; MRX96; MRXSYP; Synaptophysin; SYP; SYPH Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Mouse chimeric IgG2a