CST, 99948T, SCAMP4 (E8Y6Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SCAMP4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SCAMP4 (E8Y6Z) Rabbit Monoclonal Antibody (CST #99948) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SCAMP4 (E8Y6Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total SCAMP4 protein. Based on observed molecular weight and the sequence of the peptide antigen, this antibody is not expected to cross-react with other SCAMP family members. By western blot, this antibody recognizes a background band of unknown origin at approximately 120 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro30 of human SCAMP4 protein. Background Secretory carrier membrane proteins (SCAMPs) are a family of conserved integral membrane proteins localized to membranes of the cell-surface recycling system, including secretory organelles and endosomes. SCAMP1, SCAMP2, SCAMP3, and SCAMP4 are ubiquitously expressed, whereas SCAMP5 exhibits primarily neural expression (1,2). SCAMP4 protein is enriched on the surface of senescent cells, and its expression regulates the secretion of SASP (senescence-associated secretory phenotype) factors, including interleukin 6 (IL6), IL7, IL8, growth differentiation factor-15 (GDF-15), and C-X-C motif chemokine ligand 1 (CXCL1) (3). Alternate Names FLJ33847; FLJ90105; SCAM4; SCAMP-4; SCAMP4; Secretory carrier membrane protein 4; Secretory carrier-associated membrane protein 4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG