CST, 9996L, Oligomycin

Chemical Modulators for studying in the research area. Product Usage Information Oligomycin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 5 mg in 1.26 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 0.5 - 10 μM for 0.5 - 8 hours. Storage Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Background During oxidative phosphorylation in mitochondria, the electron transport system pumps H from the matrix into the intermembrane compartment. This process generates a H gradient across the mitochondrial inner membrane. This H gradient is used to drive mitochondrial F F ATPase to convert ADP to ATP. Oligomycin, an antibiotic from , blocks oxidative phosphorylation by binding to the F part of the ATPase to inhibit its activity (1-3). Alternate Names activator; AMPK; AMPK alpha; AMPKa; AMPKalpha; oligo; oligomycin; oxidative phosporylation; stimulator