CST, 99979S, ERAP1 (E6X1P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ERAP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ERAP1 (E6X1P) Rabbit Monoclonal Antibody (CST #99979) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ERAP1 (E6X1P) Rabbit Monoclonal Antibody recognizes endogenous levels of total ERAP1 protein. This antibody does not cross-react with ERAP2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly840 of human ERAP1 protein. Background Endoplasmic reticulum aminopeptidase 1 (ERAP1) is an IFN-γ inducible peptidase that is inserted into the endoplasmic reticulum (ER) membrane as a type II transmembrane protein (1). Research studies have shown that ERAP1 influences the adaptive branch of the immune system by facilitating the maturation of peptide fragments that are loaded onto MHC class I alleles, which are then presented for recognition by T lymphocytes (2,3). Defects in ERAP1 are associated with human disease. Multiple single nucleotide polymorphisms have been identified within , which alter the enzymatic activity of the protein product and, in conjunction with HLA-B27, confer susceptibility to ankylosing spondylitis, an inflammatory disease that affects joints (4,5). Alternate Names A-LAP; Adipocyte-derived leucine aminopeptidase; ALAP; Aminopeptidase PILS; aminopeptidase regulator of TNFR1 shedding; APPILS; ARTS-1; ARTS1; CTD-2260A17.2; Endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP; ERAAP1; ERAP1; KIAA0525; PILS-AP; PILSAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG