Merck, 3553361001, FastStart™ High Fidelity PCR System

The FastStart™ High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75 °C and are activated by heating to +95 °C for two minutes. Hot start protocols have improved specificity, sensitivity, and PCR yield. Heat-labile blocking groups on amino acid residues of FastStart™ Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically. FastStart™ Taq DNA Polymerase is activated by removal of blocking groups using a preincubation step at +95°C. The FastStart™ High Fidelity PCR System is the product of choice for multiplex PCR. •FastStart™ High Fidelity Enzyme Blend, (5 U/μl) in storage buffer •FastStart™ High Fidelity Reaction Buffer, 10x concentrated, with 18 mM MgCl₂ •FastStart™ High Fidelity Reaction Buffer, 10x concentrated, without MgCl₂ •MgCl₂ Stock Solution, 25 mM •DMSO