Merck, M5287-250G, MES hydrate

MES hydrate buffer (2-(N-morpholino)ethanesulfonic acid monohydrate) is a zwitterionic biological buffer widely employed in biological, environmental, and biochemical research. With a pKa of 6.1, it is the go-to choice for buffering solutions at physiological pH. MES was developed as one of Good′s buffers in the 1960s, meeting specific criteria: a midrange pKa, maximum water solubility, minimal solubility in other solvents, minimal salt effects, stability in different temperatures, chemical and enzymatic stability, minimal absorption in the visible and UV spectral range, and ease of synthesis. MES Monohydrate′s attributes, such as low UV absorptivity, minimal reactivity, pH stability, and water solubility, position it as a Good′s buffer. MES hydrate buffer also exhibits high water solubility and minimal metal ion binding, making it an ideal choice for various applications: Cell Biology: MES hydrate buffer is often the preferred choice for buffering cell culture media due to its lower toxicity to cells compared to other buffers like Tris and phosphate.Protein Purification and Extraction: Its stability across a wide pH range and minimal metal ion binding make MES hydrate buffer a valuable asset in protein purification and extraction protocols.Gel Electrophoresis: MES hydrate buffer is also commonly used as a running buffer in denaturing gel electrophoresis, ensuring reliable results in protein separation and analysis.