Merck, MX488AS50-1KT, Mix-n-Stain™ CF™ 488A Antibody Labeling Kit (20-50μg)

This labeling kit is for labeling 20-50 μg antibody with the green fluorescent dye CF488A. Mix-n-Stain CF dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection settings, CF488A is similar to Alexa Fluor™ 488 in brightness and photostability. In microscopy or flow cytometry applications using a 520/20 filter, CF488A may appear brighter than Alexa Fluor™ 488 due to its shorter emission wavelength, which makes the dye optically more compatible. The shorter wavelength of CF488A also minimizes the dye’s spill-over into longer wavelength channels, making the dye advantageous in multi-color detection. Like Alexa Fluor™ 488, CF488A is highly water soluble, but is not as excessively negatively charged. As a result, antibody conjugates prepared from CF488A retain excellent specificity to ensure high signal-to-noise ratio.