Merck, WGA4-50RXN, GenomePlex® Single Cell Whole Genome Amplification Kit

GenomePlex® Single Cell Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. This kit is designed to amplify the genome of a single cell. This rapid and straightforward method provides millionfold amplification yielding microgram quantities of genomic DNA from a single cell. Traditional single-cell whole genome amplification methods yield insufficient quantities with significantly biased representation. The kit includes all the reagents necessary for cell lysis and successive whole genome amplification. Single cells can be isolated by fluorescence-activated cell sorting (FACS), laser capture microdissection (LCM), dilution, or any other applicable method.