New England Biolabs, B0201S, T4 Polynucleotide Kinase Reaction Buffer

Related Categories Kinases,, Buffers Applications Phosphorylation (Kinase) Specification Unit Definition One unit of enzyme catalyzes the phosphorylation of 20 pmol of fluorescently labeled oligo in 30 min at 37˚C. 1X Buffer Components 70 mM Tris-HCl 10 mM MgCl2 5 mM DTT pH 7.6@25°C FAQ Q: What is supplied with the T4 Polynucleotide Kinase Reaction Buffer pack? A: The pack contains 4.0 ml of 10X T4 Polynucleotide Kinase Reaction Buffer. Q: What is the composition of the T4 Polynucleotide Reaction Buffer? A: 1X T4 Polynucleotide Kinase Reaction Buffer: 70 mM Tris-HCl, 10 mM MgCl2, 5 mM DTT, pH 7.6 @ 25 °C. Supplied as a 10X concentrated stock.