New England Biolabs, B0374S, Isothermal Amplification Buffer II Pack

Related Categories Reaction Buffers,, Isothermal Amplification & Strand Displacement,, Buffers Applications Isothermal Amplification,, PCR Specification 1X Buffer Components 20 mM Tris-HCl 10 mM (NH4)2SO4 150 mM KCl 2 mM MgSO4 0.1% Tween® 20 pH 8.8@25°C FAQ Q: Which enzyme should I use with Isothermal Amplifiction Buffer II? A: Isothermal Amplification Buffer II was developed for use with Bst 3.0 DNA Polymerase. It is a very similar buffer to Isothermal Amplification Buffer, but with an increased concentration of KCl for optimal Bst 3.0 reaction performance. Use with Bst and Bst 2.0 is not recommended. Q: When I thaw Isothermal Amplification Buffer II I see a lot of white precipitate, is this normal? A: Yes. Due to the high concentration of salts (MgSO4, KCl) in 10X Isothermal Amplification Buffer II, there is commonly precipitation during freeze/thaw cycles. This will easily go back into solution with vortexing, and buffer should be thoroughly vortexed before use.