New England Biolabs, B0535S, StickTogether™ DNA Ligase Buffer

Related Categories DNA Ligases,, Buffers Applications Cloning Ligation Specification 1X Buffer Components 66 mM Tris-HCl 10 mM MgCl2 1 mM ATP 1 mM DTT 7.5% Polyethylene glycol (PEG 6000) pH 7.6@25°C FAQ Q: What is supplied in the StickTogether™ DNA Ligase Buffer pack? A: The StickTogether™ DNA Ligase Buffer pack contains 2 vials, for a total of 2.0 ml of buffer. Q: The StickTogether™ DNA Ligase Buffer arrived thawed.  Are the buffer and enzyme still active? A: The buffers will often thaw when shipped on wet ice. The StickTogether™ DNA Ligase Buffer will perform optimally even if it arrives thawed. Q: Should I aliquot the StickTogether™ DNA Ligase Buffer and store it at -80°C for long-term storage? A: Aliquoting of the StickTogether™ DNA Ligase Buffer is not necessary. The recommended storage temperature for the buffer is -20°C