New England Biolabs, B7703S, Blue Protein Loading Dye

The blue protein loading dye contains one vial of blue loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications. Related Categories Protein Tools Buffers and Diluents,, Gel Loading Buffers,, Protein Standards, FAQ Q: What is supplied with the Blue Loading Buffer pack? A: The pack contains 8 ml of 3X Blue Loading Buffer and 1 ml of 30X (1.25 M) DTT. Q: What is the composition of the Blue Loading Buffer? A: 1X Blue Loading Buffer: 62.5 mM Tris-HCl (pH 6.8 @ 25°C), 2% (w/v) SDS, 10% glycerol and 0.01% (w/v) bromophenol blue. Q: How should the Blue Loading Buffer be used? A: 1.Add 1/10 volume of the 30X DTT solution to 1 volume 3X Blue Loading Buffer. 2.Add 1/2 volume of the above mix to the protein sample. 3.Heat at 100°C for 5 minutes to denature the protein. 4.Spin for 30 seconds in a microfuge to remove precipitated material. 5.Load onto gel. Q: What is the difference between the Red and Blue Loading Buffers? A: The only difference is the dye. The red was chosen for the prestained markers because the blue dye can be confused as an extra protein band. Q: What are the functions of the buffer pack components? A: DTT is present to help reduce any disulphide S-S bonds that help form secondary/tertiary structure and/or dimer formation. The SDS detergent binds to the proteins positive charges so that proteins will separate based on size and not by charge. The SDS also denatures the proteins and subunits to also help separate them based on size, not on shape.