New England Biolabs, B9035S, NEB® 10-beta/Stable Outgrowth Medium

Related Categories Competent Cells Buffers & Diluents,, Buffers FAQ Q: What competent E. coli strains should I use NEB 10-beta/Stable Outgrowth Medium with? A: We recommend the use of NEB 10-beta/Stable Outgrowth Medium with NEB 10-beta chemically competent (NEB #C3019) and electrocompetent (NEB #C3020K) E. coli, as well as with NEB Stable chemically competent E. coli (NEB #C3040). NEB 10-beta/Stable Outgrowth Medium ensure high transformation efficiency when used with these competent E. coli strains. Please do not use SOC Outgrowth Medium, as will see decreased efficiencies. Q: Can I use NEB 10-beta/Stable Outgrowth Medium with strains other than NEB 10-beta or NEB Stable Competent E.coli? A: We do not recommend the use of this outgrowth medium with strains other than NEB 10-beta and NEB Stable competent E.coli. The use of NEB 10-beta/Stable Outgrowth Medium with other competent E. coli strains can result in lower transformation efficiencies. Q: When working with NEB 10-beta Competent E.coli and NEB Stable competent E.coli, do I need to perform outgrowth dilutions prior to plating with NEB 10-beta/Stable Outgrowth Medium? A: Although we recommend the use of NEB 10-beta/Stable Outgrowth Medium for the dilution of outgrowth prior to plating when working with NEB 10-beta and NEB Stable Competent E.coli, this is not an absolute requirement. LB can also be used to dilute the outgrowth prior to plating.