New England Biolabs, B9200S, Recombinant Albumin, Molecular Biology Grade

Need to avoid Related Categories Restriction Endonuclease Buffers & Diluents,, Buffers Applications Restriction Enzyme Digestion,, Restriction Enzyme Digestion Specification Storage Buffer 20 mM Tris-HCl 100 mM KCl 0.1 mM EDTA 50% Glycerol pH 8 @ 25°C FAQ Q: What is the source of Recombinant Albumin, Molecular Biology Grade? A: This albumin is a recombinant form of human serum albumin produced in yeast. Q: What is the buffer composition of Recombinant Albumin, Molecular Biology Grade? A: The buffer composition is 20mM Tris-HCl (pH 8.0 at 25°C), 100 mM KCl, 0.1 mM EDTA and 50% glycerol. Q: Why would I want to add Recombinant Albumin, Molecular Biology Grade to my reaction? A: Adding serum albumin to a reaction can lessen enzyme loss on tube and pipette tip surfaces. Recombinant Albumin, Molecular Biology Grade offers a non-bovine alternative that behaves similarly to BSA. BSA stabilizes enzymes in reaction. The stabilizing effects are most pronounced in overnight reactions. BSA may also prevent adhesion to reaction tubes and pipette surfaces. Q: Can I use Recombinant Albumin, Molecular Biology Grade as a molecular weight marker? A: This product is not designed to be used as a molecular weight marker. Q: Can I use Recombinant Albumin, Molecular Biology Grade as a concentration standard? A: This product is not designed to be used as a concentration standard. Q: What is the difference between BSA Molecular Grade and Recombinant Albumin, Molecular Biology Grade? A: BSA, Molecular Grade (NEB #B9000) is of bovine origin. Recombinant Albumin, Molecular Biology Grade (NEB #B9200) is a non-bovine derived albumin that can serve as an alternative to BSA. Q: Is Recombinant Albumin, Molecular Biology Grade acetylated? A: No, Recombinant Albumin, Molecular Biology Grade is not acetylated.