New England Biolabs, E6056L, NEBNext® Quick Ligation Module

This module is part of the original standard library prep workflow, which is longer than the NEBNext Related Categories DNA Ligases Applications Non-Cloning Ligation Specification Materials Required but not Supplied ThermocyclerSPRISelect® Reagent Kit (Beckman Coulter® Inc. #B23317) or AMPure® XP Beads (Beckman Coulter, Inc. #A63881)10 μM Tris HCl pH 7.5-8.0 or 0.1 μM Tris HCl pH 8.0 FAQ Q: What type of sample prep/library construction can I use the Quick Ligation Module reagents for? A: The NEBNext Quick Ligation Module is compatible with protocols requiring adapter ligation of fragmented, blunt or dA tailed DNA, including Illumina’s Genomic DNA Sample Prep protocol for the Genome Analyzer II, Roche 454's Library Preparation for Standard, Titanium sequencing and Life Technologies SOLiD Library Preparation. Q: How much DNA can I blunt in one NEBNext Quick Ligation Reaction? A: Up to 5 μg of blunt or dA-Tailed DNA fragments is recommended in a single Quick Ligation reaction.