New England Biolabs, E6270L, NEBNext® Fast DNA Library Prep Set for Ion Torrent®

The NEBNext Related Categories Library Preparation for Ion Torrent Applications DNA Sequencing Specification Materials Required but not Supplied Agencourt® AMPure® XP Beads (Beckman Coulter, Inc., #A63881) or PCR Column Purification Kit (Qiagen or other) 80% ethanol (freshly prepared) Size selection materials [E-Gel® (Life Technologies, Inc.) or AMPURE XP Beads etc.] Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent) FAQ Q: What sequencing platform can I use the NEBNext Fast DNA  Library Prep Sets for Ion Torrent reagents for? A: The NEBNext Fast Library Prep Set for Ion Torrent has been validated for the Ion Torrent sequencing platform. Q: What type of starting material can be used with NEBNext® DNA Library Prep Set for Ion Torrent? A: DNA that has previously been fragmented, by mechanical or enzyme-based shearing methods. Q: How much starting material do I need to use when preparing libraries using the NEBNext Fast DNA Library Prep Set for Ion Torrent? A: 100ng –1µg. Q: Does the NEBNext DNA Library Prep Sets for Ion Torrent work well for GC-rich DNA and AT-rich DNA? A: Yes. This product can be used with DNA comprised of a broad range of GC content. Q: Can I use the NEBNext DNA Library Prep Sets for Ion Torrent reagents for preparing RNA? A: Once the RNA has been converted to dsDNA, and fragmented to the appropriate size, these reagents can be used for the remainder of the sample preparation process. Q: What methods for reaction clean up can be used with Ion Torrent Library Preparation Kits? A: Bead-based and column-based methods are compatible with this kit. Note that these reagents are not provided in this kit. Q: What methods for size selection can be used with Ion Torrent Library Preparation Kits? A: This kit and protocol is compatible with size selection using gel elution (manual or automated). Note that these reagents are not provided in this kit. Q: Are these reagents available in a customized format, larger format or 96 well formats? A: Yes, customized formats can be accommodated. Please contact our OEM department at custom@neb.com for more details.