New England Biolabs, E7180S, NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing

This product is intended for use with the original Oxford Nanopore Technologies SQK-LSK114 and Q-SQK-LSK109 protocols. The reagents required for the updated SQK-LSK114 protocols are provided in the NEBNext Companion Module v2 for Oxford Nanopore Technologies Related Categories Library Preparation for Oxford Nanopore Technologies,, Next Generation Sequencing Library Preparation FAQ Q: Which protocol should be followed when using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing A: The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use with these reagents. Q: What type and how much starting material do I need to use when preparing libraries using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing? A: 1 µg of double–stranded, high molecular weight genomic DNA (or 100-200 fmol for short-fragment libraries). Q: Are the reagents supplied in the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing sufficient to construct libraries for Oxford Nanopore technologies devices? A: No, please refer to the device-specific SQK-LSK114 protocol for information on additional reagents required. Q: Are the reagents supplied in the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing recommended for native barcoding multiplex sequencing? A: No, due to differences in protocols and volumes required, the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing is not recommended for native barcoding. Please consult the appropriate native barcoding protocol on the Oxford Nanopore Technologies website for appropriate reagent recommendations. Q: What is the difference between NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7672) & NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7180)? A: The NEBNext Companion Module v2 for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7672) contains updated reagents to reflect changes in many of Oxford Nanopore Technologies updated SQK-LSK114 protocols. The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use with these reagents. These protocols can be downloaded from the ONT Community pages. Q: Can I still use the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7180) for SQK-LSK114 sequencing? A: Please check the protocol you are following. Previous protocol versions can be downloaded from the Nanopore Community Site. If that protocol recommends use of the (NEB #E7180) kit, then you can continue to use this module for the protocol. However, for optimal performance we recommend following Oxford Nanopore's most up-to-date protocols and reagent recommendations.