New England Biolabs, E7490L, NEBNext® Poly(A) mRNA Magnetic Isolation Module

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails. Related Categories RNA Depletion & mRNA Enrichment,, RNA Library Prep for Illumina,, Next Generation Sequencing Library Preparation Specification Materials Required but not Supplied 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube. Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent) Thermal cycler or heat block Bioanalyzer® (Agilent Technologies, Inc.) or similar instrument and consumables. FAQ Q: Can I isolate poly(A)+ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)? A: No, this kit is for isolation of mRNA from previously isolated Total RNA. Q: Do I need two rounds of binding? A: Yes, we recommend two RNA binding events to the beads to recover higher yields of mRNA. Q: Can I use the Oligo (dT)25 Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications? A: No. We recommend using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490) for NGS applications.