New England Biolabs, E7550S, NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

Related Categories Automation for NEBNext® NGS Library Prep,, RNA Library Prep for Illumina,, Next Generation Sequencing Library Preparation Specification Materials Required but not Supplied Magnetic rack or plate (e.g., NEBNext ® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent) FAQ Q: What is the difference between the NEBNext Ultra II Directional RNA Second Strand Module and the NEBNext mRNA Second Strand Synthesis Module? A: The NEBNext Ultra II Directional RNA Second Strand Module includes the Second Strand Synthesis Reaction Buffer with dUTP Mix (dATP, dCTP, dGTP, and dUTP). The dUTP is used for labeling of the second strand for strand-specific library preparation by the Ultra Directional RNA method. The NEBNext mRNA Second Strand Synthesis Module contains Second Strand Synthesis Buffer that contains dATP, dCTP, dGTP and dTTP, and is not compatible with the Ultra Directional workflow. Q: For the downstream workflow, which Modules for End Repair, dA-Tailing and Ligation should be used with the NEBNext Ultra Directional Second Strand Synthesis Module? A: This module is compatible with the NEBNext Ultra End Repair/dA-Tailing Module (#E7442) and the NEBNext Ultra Ligation Module (#E7445).