New England Biolabs, E7595S, NEBNext® Ultra™ II Ligation Module 

This module is part of the Ultra Related Categories DNA Library Prep for Illumina,, RNA Library Prep for Illumina,, Next Generation Sequencing Library Preparation Specification Materials Required but not Supplied Magnetic rack or plate (e.g., NEBNext ® Magnetic Separation Rack (NEB #S1515S) or Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent) Multiplex Oligos (Neb.com/oligos) NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or NEBNext Ultra II FS DNA Module (NEB #E7810) and associated reagents and consumables. NEBNext Ultra II Q5 ® Master Mix (NEB #M0544) FAQ Q: What is the difference between the three NEBNext Ligation Modules? A: There are 3 NEBNext Ligation modules: 1. The NEBNext Ultra II Ligation Module (#E7595) contains the ligation components designed to be used with the new Ultra II kit workflow, and should be used downstream of the NEBNext Ultra II End Repair/dA-tailing Module (#E7546). If used as designed, cleanup of the end repair/dA-tailing reaction is not required prior to use with the Ultra II Ligation Module. This combination of modules (#E7595 and #E7546) gives you the highest library yields and a simplified workflow. 2. The NEBNext Ultra Ligation Module (#E7445) contains the ligation components designed to be used with the Ultra kit workflow, and should be used downstream of the NEBNext Ultra End Repair/dA-tailing Module (#E7442). If used as designed, cleanup of the end repair/dA-tailing reaction is not required prior to use with the Ultra Ligation Module. This combination of modules (#E7445 and #E7442) gives you high library yields from a simplified workflow. 3. NEBNext Quick Ligation Module (#E6056) contains the ligation components designed to be used the NEBNext End Repair Module (#E6050) and the NEBNext dA-Tailing Module (#E6053). This combination of modules (#E6056, #E6053, and #E6050) gives you high library yields from a standard library construction workflow. Q: How do I obtain the NEBNext Adaptor and USER enzyme for use with the NEBNext Ultra II Ligation Module? A: The NEBNext Ultra II Ligation Module is compatible with the NEBNext Singleplex or Multiplex Oligos for Illumina (neb.com/oligos), which contain the adaptor, USER enzyme as well as the necessary primers required for PCR. The NEBNext Ultra II Ligation Module is also compatible with other adaptors suitable for the Illumina sequencing platform.