New England Biolabs, E7672L, NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing

This new module contains the NEBNext DNA repair, end repair and ligation reagents recommended for Oxford Nanopore Technologies updated SQK-LSK114 singleplex ligation sequencing library prep protocols. Related Categories Library Preparation for Oxford Nanopore Technologies,, Next Generation Sequencing Library Preparation FAQ Q: What is the difference between NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7672) & NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7180)? A: The NEBNext Companion Module v2 for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7672) contains updated reagents to reflect changes in many of Oxford Nanopore Technologies updated SQK-LSK114 protocols. The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use with these reagents. These protocols can be downloaded from the ONT Community pages. Q: Is the NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing the best choice if I am performing native barcoding ONT sequencing? A: No, the reagent volumes included in this module are designed to support singleplex SQK-LSK114 protocols. If you are performing multiplexing using native barcoding then please use the individual reagents as recommended in the ONT protocols. Q: Which protocol should be followed when using the NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing? A: The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use with these reagents. These protocols can be downloaded from the ONT Community pages.