New England Biolabs, M0236S, T4 Polynucleotide Kinase (3' phosphatase minus)

Related Categories Kinases,, RNA Modification Applications Phosphorylation (Kinase) Specification Unit Definition One unit of enzyme catalyzes the phosphorylation of 20 pmol of fluorescently labeled oligo in 30 min at 37˚C. Reaction Conditions 1X T4 Polynucleotide Kinase Reaction Buffer Incubate at 37°C 1X T4 Polynucleotide Kinase Reaction Buffer 70 mM Tris-HCl 10 mM MgCl2 5 mM DTT (pH 7.6 @ 25°C) Usage Concentration 10,000 units/ml Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1 µM ATP pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes Unit Assay Conditions 1X T4 Polynucleotide Kinase Reaction Buffer, 66 µM [γ -32 P] ATP (5 x 10 6 cpm/µmol), 0.26 mM 5´-hydroxyl-terminated salmon sperm DNA. FAQ Q: Will T4 PNK (3' phosphate minus) work in rCutSmart Buffer? A: T4 PNK (3' phosphate minus) will work in rCutSmart Buffer, with the addition of ATP and DTT. To see its % functional activity in rCutSmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.