New England Biolabs, M0243S, RNase If

RNase I Related Categories RNases,, Epitranscriptome Analysis Specification Unit Definition One unit is defined as the amount of enzyme required to fully digest 1 picomole of synthetic ssRNA 33-mer in a total reaction volume of 10 μl in 15 minutes in 1X NEBuffer 3 as visualized on a 20% acrylamide gel. Reaction Conditions 1X NEBuffer™ 3 Incubate at 37°C 1X NEBuffer™ 3 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.5 mM EDTA 50% Glycerol pH 8 @ 25°C Heat Inactivation 70°C for 20 minutes