New England Biolabs, M0245L, ShortCut® RNase III

Related Categories RNases,, Epitranscriptome Analysis Specification Unit Definition One unit is the amount of enzyme required to digest 1 µg of fluorescently labelled 40 bp dsRNA to siRNA in 20 minutes at 37°C in a total reaction volume of 20 μl. Reaction Conditions 1X ShortCut Reaction Buffer Incubate at 37°C 1X ShortCut Reaction Buffer 50 mM Tris-HCl 1 mM DTT 50 mM NaCl (pH 7.5 @ 25°C) Usage Concentration 1X Storage Buffer 500 mM NaCl 10 mM Tris-HCl 0.5 mM EDTA 1 mM DTT 50% Glycerol pH 8 @ 25°C Heat Inactivation No Unit Assay Conditions One unit is the amount of enzyme required to digest 1 μg of dsRNA to siRNA in 20 minutes at 37°C in a total reaction volume of 50 μl.