New England Biolabs, M0247S, Micrococcal Nuclease

Related Categories Chromatin Analysis,, Exonucleases and Non-specific Endonucleases,, RNA Modification Specification Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 15 minutes at 37°C, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Reaction Conditions 1X Micrococcal Nuclease Reaction Buffer Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X Micrococcal Nuclease Reaction Buffer 50 mM Tris-HCl 5 mM CaCl2 (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM NaCl 1 mM EDTA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation No FAQ Q: Will Micrococcal Nuclease cleave DNA as well as RNA and what type of end is left after digestion? A: The enzyme cleaves DNA as well as RNA, and leaves a 3’-P and 5’-OH end. Q: Does Micrococcal Nuclease work in ChIP sequencing protocols? A: Yes, Micrococcal Nuclease works well with the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 from Cell Signaling Technology. Please follow the link to see product information and protocols on their website. Q: Will Micrococcal Nuclease work in rCutSmart Buffer? A: Micrococcal nuclease will work in rCutSmart Buffer, with the addition of calcium. To see its % functional activity in rCutSmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.