New England Biolabs, M0265L, Exonuclease T

Related Categories RNases,, Epitranscriptome Analysis,, Exonucleases and Non-specific Endonucleases Specification Unit Definition One unit is defined as the amount of enzyme required to release 1 nmol of single dT nucleotides from 50 pmol of Fam-labeled polythymidine substrate in 30 minutes at 25°C. Reaction Conditions 1X NEBuffer™ 4 Incubate at 25°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C) Activity in NEBuffers Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes FAQ Q: Is Exonuclease T salt tolerant? A: We recommend maintaining the reaction conditions below 50 mM salt.