New England Biolabs, M0277S, AMV Reverse Transcriptase

Related Categories RT-PCR,, cDNA Synthesis & Reverse Transcriptases Applications Transcription-Mediated and NASBA Amplification,, cDNA Synthesis,, Reverse Transcription (cDNA Synthesis), Specification Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37°C using poly(rA)-oligo(dT) as template primer. Reaction Conditions 1X AMV Reverse Transcriptase Reaction Buffer Incubate at 42°C 1X AMV Reverse Transcriptase Reaction Buffer 50 mM Tris-acetate 75 mM Potassium Acetate 8 mM Magnesium Acetate 10 mM DTT (pH 8.3 @ 25°C) Storage Buffer 200 mM potassium phosphate 2 mM DTT 50% Glycerol 0.2% Triton® X-100 pH 7.2 @ 25°C Heat Inactivation 85°C for 5 minutes Unit Assay Conditions 75 mM potassium acetate, 50 mM Tris-HCl (pH 8.3) 8 mM magnesium acetate, 0.5 mM [ 3 H]-dTTP, 0.2 mM poly(rA)•oligo(dT)12–18 and 10 mM DTT.