New England Biolabs, M0296S, Thermostable Inorganic Pyrophosphatase

Related Categories Phosphatases,, RNA Modification Specification Unit Definition One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl 2 , 0.32 mM PPi, reaction volume of 0.5 ml). Storage Buffer 20 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 8 @ 25°C Heat Inactivation No FAQ Q: What is the activity of TIPP at 37°C? A: This enzyme has 1% of its activity at 37°C.