New England Biolabs, M0313S, hAAG

Related Categories DNA Repair Enzymes and Structure-specific Endonucleases Specification Unit Definition One unit is defined as the amount of enzyme required to create an AP site from 1 pmol of a 34-mer oligonucleotide duplex containing a single deoxyinosine site in a total reaction volume of 10 μl in 1 hour at 37°C. Reaction Conditions 1X ThermoPol® Reaction Buffer Incubate at 37°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) Storage Buffer 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.5% Tween® 20 0.5% IGEPAL® CA-630 pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes Molecular Weight Theoretical: 25752 daltons Unit Assay Conditions 1X ThermoPol Buffer containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 μl. FAQ Q: What substrate is used to test hAAG? A: The substrates we use are listed under the unit definition for each enzyme. hAAG is tested using oligos that have a deoxyinosine base incorporated, which is a standard modification that companies such as IDT can provide. Q: What is the molecular weight of hAAG? A: The calculated molecular weight of hAAG is 25.75 kDa. Q: Is hAAG a tagged protein? A: No. hAAG is not a tagged protein.