New England Biolabs, M0327S, Sulfolobus DNA Polymerase IV

Related Categories DNA Manipulation Applications PCR Specification Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 55°C. Reaction Conditions 1X ThermoPol® Reaction Buffer Incubate at 55°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) Storage Buffer 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation No Molecular Weight Theoretical: 40000 daltons 5' - 3' Exonuclease No 3' - 5' Exonuclease No Strand Displacement No Unit Assay Conditions 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13mp18. Activity at Temperature @30°C: 5% @37°C: 20% @45°C: 40% @65°C: 90% @72°C: 70%