New England Biolabs, M0330L, Bsu DNA Polymerase, Large Fragment

Optimal performance in a number of applications Related Categories DNA Manipulation Applications Recombinase Polymerase Amplification and SIBA,, Polymerases for DNA Manipulation,, RT-PCR & cDNA Synthesis, Specification Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. Reaction Conditions 1X NEBuffer™ 2 Incubate at 37°C 1X NEBuffer™ 2 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) Heat Inactivation 75°C for 20 minutes 5' - 3' Exonuclease No 3' - 5' Exonuclease No Strand Displacement + Unit Assay Conditions 1X NEBuffer 2, 33 µM dNTPs including [ 3 H]-dTTP and 70 µg/ml denatured herring sperm DNA.