New England Biolabs, M0331S, 5´ Deadenylase

5´ Deadenylase is capable of deadenylation from 5´-end of DNA and RNA, leaving the phosphate at 5´-end. It also cleaves AppppA into ATP and AMP. Related Categories RNA Modification Applications RNA Cloning Specification Unit Definition One unit is defined as the amount enzyme required to remove 10 pmoles of AMP from a 5'-adenylated DNA oligo in 10 minutes at 30°C. Reaction Conditions 1X NEBuffer™ 1 Incubate at 30°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C) Storage Buffer 10 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% (w/v) Triton® X-100 pH 7.5 @ 25°C Heat Inactivation 70°C for 20 minutes FAQ Q: What is the molecular weight of 5’ Deadenylase? A: The molecular weight of 5’ Deadenylase is 26 kDa. Q: Is 5’ Deadenylase a tagged protein? A: No. 5’ Deadenylase is not a tagged protein. Q: Can 5’ Deadenylase deadenylate ssDNA? A: Yes. 5’ Deadenylase will deadenylate 5’-adenylated dsDNA, ssDNA and nicked DNA. Deadenylation is more efficient on dsDNA and nicked DNA substrates.