New England Biolabs, M0336S, hSMUG1

Related Categories DNA Repair Enzymes and Structure-specific Endonucleases Specification Unit Definition One unit is defined as the amount of enzyme required to excizse 1 pmol of deoxyuracil from a 34 mer oligonucleotide duplex containing a single dU site in a total reaction volume of 10 μl in 1 hour at 37°C. Reaction Conditions 1X NEBuffer™ 1 Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C) Storage Buffer 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes Molecular Weight Theoretical: 29861 daltons Unit Assay Conditions 1X NEBuffer 1 supplemented with 100 µg/ml Recombinant Albumin containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 μl. After release of dU, the oligonucleotide is cleaved by treating the resulting AP site with 100 mM NaOH for 10 minutes at 80°C. FAQ Q: What is the activity of hSMUG1 in the NEBuffers 1-4? A: hSMUG1 has full activity in NEBuffer 1, NEBuffer r1.1, NEBuffer 4 and rCutSmart. It has 25% activity in NEBuffer 3. Its use in NEBuffer 2 is not recommended. Q: What is the molecular weight of hSMUG1? A: The molecular weight of hSMUG1 is 29.86kDa. Q: What substrate is used to test hSMUG1? A: The substrates used to test enzyme activity are listed under the unit definition for each enzyme. hSMUG1 is tested using a 34-mer oligonucleotide containing a single dU site. Q: Is hSMUG1 a tagged protein? A: No. hSMUG1 is not a tagged protein.