New England Biolabs, M0361L, Pyrophosphatase, Inorganic (E. coli)

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. Related Categories Phosphatases,, RNA Modification,, RNA Synthesis In vitro Transcription (IVT), Specification Unit Definition One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl 2 and 2 mM PPi). Storage Buffer 20 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 8 @ 25°C Heat Inactivation No