New England Biolabs, M0398S, Apyrase

Apyrase (recombinant, Related Categories Phosphatases,, RNA Modification Specification Unit Definition One unit is defined as the amount of enzyme that catalyses the release of 1 μmol of inorganic phosphate from ATP (1 mM, NEB #P0756 ) in 1X Apyrase Reaction Buffer in 1 minute at 30°C in a total reaction of 50 μl. Reaction Conditions 1X Apyrase Reaction Buffer Incubate at 30°C 1X Apyrase Reaction Buffer 20 mM MES 50 mM NaCl 5 mM CaCl2 1 mM DTT 0.05% Tween® 20 (pH 6.5 @ 25°C) Storage Buffer 20 mM MES 50 mM NaCl 0.1 mM CaCl2 1 mM DTT 0.1% Tween® 20 50% Glycerol pH 6.5 @ 25°C Heat Inactivation 65°C for 20 minutes Molecular Weight Apparent: 47 kDa Specific Activity 3,000 units/mg FAQ Q: What is the activity of Apyrase in other NEBuffers? A: E. coli Apyrase retains approximately 30% activity in NEBuffers r1.1, r2.1, r3.1, and rCutSmart™. Q: Can Mg2+ be substituted for Ca2+ in Apyrase reaction buffer? A: E. coli Apyrase is approximately 50% active when Mg2+ substitutes Ca2+ in Apyrase Reaction Buffer.