New England Biolabs, M0628S, RNase Inhibitor, Murine (Glycerol-free)

RNase Inhibitor, Murine, specifically inhibits RNases A, B and C. Related Categories RNase Inhibitors,, Nucleotide Solutions for RNA,, NEBExpress, ®, Cell-free, E. coli, Protein Synthesis System, Applications NEBExpress® Cell-free E. coli Protein Synthesis System,, PURExpress,, RNA Cloning, Specification Unit Definition One unit is defined as the amount of Murine RNase Inhibitor (Glycerol-free) required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A. FAQ Q: How many freeze/thaw cycles can RNase Inhibitor, Murine (Glycerol-free) tolerate? A: RNase Inhibitor, Murine (Glycerol-free) was tested up to 10 freeze/thaws at 40,000 U/ml and showed no activity loss. However, repeated freezing and thawing of this product should be avoided. The enzyme may be stored at 4°C for up to one week. Q: What is the difference between RNase Inhibitor, Murine, (NEB #M0314) and RNase Inhibitor, Murine (Glycerol-free), (NEB #M0628)? A: M0314 and M0628 have slight differences in formulation, including the removal of glycerol in M0628. Q: Can I use RNase Inhibitor, Murine (Glycerol-free) in PURExpress® In vitro Protein Synthesis and NEBExpress Cell-free E. coli Protein Synthesis workflows? A: Yes, with one exception. We do not recommend using RNase Inhibitor, Murine (Glycerol-free) with the PURExpress Δ (aa, tRNA) Kit (NEB #E6840S) due to slight variations in the enzyme’s formulation that may interfere with this kit.