New England Biolabs, M0651S, TelN Protelomerase

GMP-grade reagent also available. Related Categories Other Specification Unit Definition One unit is defined as the amount of enzyme required to cleave 0.5 μg of pMiniT-TelN BsaI-linearized control plasmid (313 fmol of TelN recognition sites) in a total reaction volume of 50 μl in 30 minutes at 30°C in 1X ThermoPol Reaction Buffer. Reaction Conditions 1X ThermoPol® Reaction Buffer Incubate at 30°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) Heat Inactivation 75°C for 5 minutes FAQ Q: What is the structure at the cleavage site of the dsDNA after TelN Protelomerase digestion? A: Unlike the cleavage ends generated by most restriction enzymes, the ends generated by TelN Protelomerase are neither sticky nor blunt. The ends have hairpin structures with covalently closed ends that are resistant to digestion by exonucleases (e.g. Lambda Exonuclease, M0663 T5 Exonuclease, Exonuclease V, Exonuclease III, Exonuclease VIII (truncated) and T7 Exonuclease). Q: Does TelN Protelomerase digest TelN recognition sequences in supercoiled dsDNA (e.g. plasmid) and/or linear dsDNA? A: Yes, TelN Protelomerase will digest both supercoiled and linear dsDNA carrying TelN recognition sites. Q: What is the activity of TelN Protelomerase in common buffers? A: Buffer % Activity Thermopol Buffer 100 NEB phi29 DNA Polymerase Reaction Buffer 25 Qiagen phi29 Buffer < 1 NEBuffer 1.1 50 NEBuffer 2.1 25 NEBuffer 3.1 < 1 Cutsmart Buffer 50 Taq DNA Polymerase Reaction Buffer 50 IsoAmp Reaction Buffer 50 LongAmp Taq Reaction Buffer 50 Q5 High-Fidelity DNA Polymerase Buffer < 1 Phusion HF reaction Buffer 12.5 Tris-HCl (pH 7.5) 50 Tris-HCl (pH 8) 75 Tris-HCl (pH 8.3) 50 Tris-HCl (pH 8.8) 75 Tricine (pH 8.7) 50 Q: Can I purchase large amounts of an existing Enzyme for Innovation? A: Yes, if you have use for large amounts of an Enzyme for Innovation, please contact NEB at EnzymesForInnovation@neb.com. Please note that there may be limited availability and thus extended lead times required for large orders. Additional QC testing may also be necessary to meet your specific application and needs. Q: What are Enzymes for Innovation? A: Enzymes for Innovation (EFI) is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities that are not commercially available elsewhere at the quality that you would expect from NEB. Enzymes for Innovation graduates are enzymes that have transitioned from having unknown or exploratory applications to becoming the cornerstone of a defined application. If you have an idea for an “Enzyme for Innovation” with a suggested application that may be useful, please email us at EnzymesForInnovation@neb.com. For more information, please view video.