New England Biolabs, M2403L, Pyrophosphatase, Inorganic (yeast)

Related Categories Phosphatases,, RNA Modification,, RNA Synthesis In vitro Transcription (IVT), Applications Whole Genome Amplification & Multiple Displacement Amplification Specification Unit Definition One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl 2 and 2 mM PPi in a reaction volume of 0.5 ml). Storage Buffer 20 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 8 @ 25°C Molecular Weight Theoretical: 71 kDa FAQ Q: Can alternate metals be used as activators for Yeast Inorganic Pyrophosphatase? A: Enzyme activity requres Mg2+ for optimal activity, although alternate metals can also act as activators: Mg2+>Zn2+>Co2+>Mn2+>Ca2+ (3).