New England Biolabs, N0341S, Lambda PFG Ladder

Convenient size markers for pulsed-field gel electrophoresis (PFG) Related Categories DNA Markers & Ladders Applications DNA Analysis Specification Bases Fragment bp 1 1018500 2 970000 3 921500 4 873000 5 824500 6 776000 7 727500 8 679000 9 630500 10 582000 11 533500 12 485000 13 436500 14 388000 15 339500 16 291000 17 242500 18 194000 19 145500 20 97000 21 48500 Effective Size Range 48,500bp to 1,018,500bp Storage Buffer 10 mM Tris-HCl 1 mM EDTA 50% Glycerol 1% LMP agarose pH 8 @ 25°C FAQ Q: Why am I seeing poor results when running the PFG marker on my pulse field gel? A: There are several factors that lead to poor results for PFG markers. Here is a list of recommendations for ensuring a sharp high quality banding pattern: Use Chef DR-II system with a hexagon grid 120 degrees (do not use field inversion, 180 degrees). Use fresh 0.5X TBE for the running buffer and the gel. Use high quality water (Milli Q) for making the buffer and the gel. Load the marker as solid plug (never melt the plug). Load a thin slice of the marker, about 1 gradation, on the syringe (a thick plug will overload the well with DNA and lead to smeared blurry pattern). Always run the recommended program for each specific marker. The programs are optimized for resolving bands in the size range corresponding to each marker. Interchanging programs with different markers will often result in a poor resolution. Store the marker at -20°C.