New England Biolabs, N0363S, dsRNA Ladder

Related Categories RNA Markers & Ladders,, RNA Markers and Ladders Specification Bases Fragment bp 1 500 2 300 3 150 4 80 5 50 6 30 7 21 Effective Size Range 21bp to 500bp Storage Buffer 10 mM Tris-HCl 1 mM EDTA pH 8 @ 25°C FAQ Q: What types of gels are recommended for ssRNA and dsRNA ladders? (N0363, N0362, N0364) A: In general, when working with single-stranded RNA molecules, one should use a denaturing gel, a denaturing sample loading buffer/dye containing either formamide or urea, and SYBR Gold for staining. The SYBR Gold stain binds preferentially to single-stranded molecules and therefore it give good visualization of the bands after gel electrophoresis. In some instances, when ssRNA molecules are large in size such as the ssRNA Ladder (N0362S), one can use a native gel and stain the gel with ethidium bromide. But keep in mind that the ssRNA samples must always be prepared using a denaturing sample loading buffer/dye containing either formamide or urea. When dealing with double-stranded RNA molecules, one only needs to use a native gel, a non-denaturing sample loading buffer/dye, and stain the gel with either ethidium bromide, SYBR Green or SYBR Safe. Q: What types of gel and staining dyes can I use for NEB’s dsRNA Ladder (N0363S)? A: One definitely needs to use a native gel such as 2.0% agarose-TBE as recommended in the protocol to run the dsRNA Ladder. The ladder is prepared for gel electrophoresis using a RNase-free, non-denaturing sample loading buffer/dye (similar to those that are used for preparing DNA samples). The gel is then stained with either ethidium bromide, SYBR Green or SYBR Gold.