New England Biolabs, N0433S, Pseudouridine-5´-Triphosphate (Pseudo-UTP)

Related Categories Nucleotide Solutions for RNA,, Nucleotide Solutions,, RNA Modification, FAQ Q: Can modified ribonucleotides be used with HiScribe® T7 High Yield RNA Synthesis Kit (NEB #E2040)? A: Yes. Modified ribonucleotides are compatible with the following NEB products: HiScribe T7 High Yield RNA Synthesis Kit (NEB #E2040S, #E2040L) HiScribe Quick T7 High Yield RNA Synthesis Kit (NEB #E2050S, #E2050L) (partial substitution only as the included NTPs are master mixed) HiScribe T7 ARCA mRNA Kit (with tailing) (NEB #E2060S) HiScribe T7 ARCA mRNA Kit (NEB #E2065S) HiScribe SP6 RNA Synthesis Kit (NEB #E2070S) HiScribe T7 High Yield RNA Synthesis with CleanCap® Reagent AG (NEB #E2080S, #E2080L) Q: Can I completely substitute a modified NTP for the canonical NTP counterpart? Will complete substitution impact yield? A: Yes. Complete substitution of modified NTPs for the canonical NTP is possible. This may slightly reduce IVT yield compared to reactions using only canonical NTPs. Minimum yield for NEB RNA synthesis kits will be achieved with complete substitution of modified NTPs. Q: Can I use more than one modified NTP in the same IVT? Will this impact yield? A: Yes. Using multiple modified NTPs in the same IVT is possible. This may slightly reduce IVT yield compared to reactions using only canonical NTPs. Minimum yield for NEB RNA synthesis kits will be achieved with complete substitution of modified NTPs.