New England Biolabs, N0445S, 7-deaza-dGTP

Related Categories Nucleotide Solutions Applications PCR,, PCR FAQ Q: What is 7-deaza-dGTP used for? A: 7-deaza-dGTP can be substituted for dGTP when sequencing GC rich regions. This helps prevent compressions. 7-deaza-dGTP makes one less chemical contact than dGTP and acts more like dATP in sequencing reactions. Q: How stable are the nucleotides? A: When stored at -20°C, they are stable for years. If they are freeze thawed frequently they should be stable for a year. If the solutions are stored at 4°C bacteria can grow in them. Bacterial growth is accelerated if the solution is diluted in a buffer containing magnesium. Discard solutions stored at 4°C after one week.