New England Biolabs, N3033S, pBR322 Vector

Related Categories DNA Plasmids & Substrates Specification Copy Number Medium (10-100) Origin of Replication pMB1 Storage Buffer 10 mM Tris-HCl 1 mM EDTA pH 8 @ 25°C Molecular Weights Apparent: 2.83 daltons Theoretical: 2.83 daltons FAQ Q: How homogeneous is the DNA/ how much is supercoiled? A: We do not guarantee a specific level of supercoiled (RFI) for any plasmid but most are at or above 90% supercoiled monomer, with minor bands of either dimeric or nicked plasmid visible in some lots. Upon linearization, the only significant band is the single, monomeric, linear form of the plasmid. Q: Are the plasmids cesium purified? A: No, we use a silica binding process as their primary purification step, with enzymatic treatment of the plasmids performed as needed to eliminate traces of genomic or single-stranded DNA. Q: What is the DNA sequence of this product? A: The DNA Sequence can be found in the DNA Sequences and Maps Tool.