New England Biolabs, P0763S, E. coli Ribosome

The Related Categories PURExpress® In Vitro Protein Synthesis,, Cell-Free Protein Expression,, Protein Expression Applications PURExpress,, Cell-Free Protein Expression,, Protein Expression Specification Heat Inactivation No FAQ Q: What is the storage buffer for the E. coli ribosomes? A: The components of the buffer are as follows:20 mM Hepes-KOH, pH 7.610 mM Mg(OAc)230 mM KCl7 mM beta-mercaptoethanol Q: Are the ribosomes the same as what is provided in the PURExpress Protein Synthesis kits? A: This ribosome preparation is highly active in NEB’s PURExpress Protein Synthesis reaction and is equivalent to the control ribosomes included in the PURExpress Δ Ribosome Kit (E3313S). Q: I want to work with the 50S or 30S ribosomal subunits. Are the 50S and 30S ribosomal subunits available dissociated/separately? A: The ribosome is supplied as the intact 70S unit. In order to work with the individual 30S and/or 50S subunits they would have to be dissociated and purified. References describing this can be found in the literature. An example of a typical protocol can be found in the following paper:Moazed D, Stern S, Noller HF. Rapid chemical probing of conformation in 16 S ribosomal RNA and 30 S ribosomal subunits using primer extension. J Mol Biol. 1986 Feb 5;187(3):399-416. PubMed PMID: 2422386 Q: How many PURExpress reactions in one pack of E. coli ribosome P0763S? A: Although this ribosome preparation is highly active in NEB’s PURExpress Protein Synthesis Kit (NEB #E6800) it is intended as a source of ribosomes only. We supply enough ribosome for approximately 6 E3313S standard PURExpress reactions. For cases where in vitro transcription and translation will be performed exclusively using PURExpress components, the E6800 kit is more cost effective and easier to use. Please refer to the E3313S and E6800S manuals for a complete description of the kit and related product information. Q: Where can I find many more detailed FAQs for PURExpress? A: Please click for the complete set of FAQs for PURExpress.