New England Biolabs, P8070S, Enterokinase, light chain

Related Categories Proteases Applications Fusion Protein Cleavage,, Protein Purification,, Protein Digestion Specification Unit Definition 1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25°C in a total reaction volume of 25 μl. Removal Enterokinase will bind specifically to trypsin inhibitor agarose. Storage Buffer 20 mM Tris-HCl 200 mM NaCl 2 mM CaCl2 50% Glycerol pH 7.2 @ 4°C Molecular Weights Apparent: 31 kDa Theoretical: 26.3 kDa Unit Assay Conditions Two fold dilutions of Enterokinase, light chain are incubated with 25 µg MBP-EK-paramyosin-ΔSal substrate in 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2 (pH 8.0 @ 25°C) in a 25 μl reaction. The reaction mix is incubated at 25°C for 16 hours. Separation of reaction products are visualized by SDS-PAGE.