New England Biolabs, P8077L, Furin

Furin is a recombinant, ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg˅. However, the enzyme prefers the site Arg-X-Lys/Arg-Arg˅. Related Categories Proteases Applications Fusion Protein Cleavage,, Protein Purification,, Protein Digestion Specification Unit Definition 1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 μl. Storage Buffer 10 mM MES 1 mM CaCl2 50% Glycerol pH 7 @ 25°C Molecular Weight Theoretical: 52.7 kDa Unit Assay Conditions Two fold dilutions of Furin are incubated with 25 µg MBP-FN-paramyosin-ΔSal substrate in 20 mM HEPES, 0.1% Triton X-100, 1 mM CaCl 2, 0.2mM β-mercaptoenthanol (pH 7.5 @ 25°C) in a 25 μl reaction. The reaction mix is incubated at 25°C for 6 hours. Separation of reaction products are visualized by SDS-PAGE.