New England Biolabs, R0138T, SalI

The large concentrated size of this product was discontinued on December 15, 2025. The small concentrated size will remain available. Related Categories Restriction Endonucleases S,, Time-Saver Qualified Restriction Enzymes Applications Fast Cloning: Accelerate your cloning workflows with reagents from NEB,, Restriction Enzyme Digestion Specification Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 μl. Reaction Conditions 1X NEBuffer™ r3.1 Incubate at 37°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Activity in NEBuffers NEBuffer™ r1.1: <10% NEBuffer™ r2.1: <10% NEBuffer™ r3.1: 100% rCutSmart™ Buffer: <10% Diluent Compatibility Diluent A Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 300 µg/ml BSA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 65°C for 20 minutes Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked FAQ Q: Is SalI affected by methylation? A: SalI is blocked by CpG methylation. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE. Q: Is SalI sensitive to pH? A: SalI activity decreases if the reaction buffer pH is not between 7.8 and 8.0. Q: Is SalI affected by star activity? A: Yes, high glycerol, high enzyme concentration, or long reaction times can cause star activity of SalI. Q: Does SalI exhibit reduced activity on supercoiled DNA? A: Supercoiled plasmids may require up to 10-fold more SalI for complete digestion than on linear DNA. Q: How many bases does SalI require for cleavage close to the ends? A: When cleaving close to the ends of DNA fragments, cleavage should be done at 37°C for one hour using 10 units/μg of DNA with a minimum of 3 bases on each side of the recognition sequence. Q: What is the molecular weight of SalI? A: 30 kDa. Q: Are more units of SalI required to cut supercoiled DNA than lambda DNA? A: Supercoiled limus, pBR322 and pUC plasmids require up to 10 times more enzyme to achieve complete digestion. Q: What is the activity of SalI at 25°C? A: It is 50-75% active. Q: Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer? A: NEB's Time-Saver™ enzymes have the benefit of working fast (5-15 minutes), but are also designed and qualified to withstand overnight digestions without degradation of DNA. Q: Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)? A: All HF-restriction enzymes and most NEB non-HF restriction enzymes are supplied with Gel Loading Dye, Purple (6X). The non-HF restriction enzymes that come supplied with purple dye are: AatII AsiSI BsmBI-v2 EagI MboI NlaIII PvuI SmaI Acil AvrII BspHI EcoRI MboII NotI RsaI SpeI AfeI BamHI BsrGI EcoRV MluI NspI SacI StuI AflII BbsI ClaI Esp3I FseI MseI PacI SacII AgeI BglII DdeI HaeIII MspI PciI SalI XbaI AluI BsaI DpnI HindIII NcoI PmeI SapI XhoI ApaI BseYI DpnII HpaI NdeI PsiI-v2 SfaNI XmaI ApeKI BsiWI DraI KpnI NheI PstI SfiI XmnI AscI * All HF restriction enzymes are also supplied with Gel Loading Dye, Purple (6X) Q: Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers? A: NEB is excited to announce that we have switched BSA-containing reaction buffers (NEBuffer 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We are also in the process of moving all restriction enzyme formulations to contain rAlbumin. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price.