New England Biolabs, R0696S, PI-SceI

The large size of this product was discontinued on 12/31/2020. The small size will continue to be available. Related Categories Homing Endonucleases,, Restriction Endonucleases P R Applications Restriction Enzyme Digestion Specification Unit Definition One unit is defined as the amount of enzyme required to cleave 1 μg of pBSvdeX XmnI-linearized Control Plasmid in 3 hours at 37°C in a total reaction volume of 50 μl. Reaction Conditions 1X NEBuffer™ PI-SceI Supplement with Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ PI-SceI 10 mM MgCl2 1 mM DTT 10 mM Tris-HCl 100 mM KCl (pH 8.6 @ 25°C) Activity in NEBuffers NEBuffer™ r1.1: 10% NEBuffer™ r2.1: 10% NEBuffer™ r3.1: 10% rCutSmart™ Buffer: 10% Diluent Compatibility Diluent B Storage Buffer 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 500 µg/ml BSA 50% Glycerol 300 mM NaCl pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes FAQ Q: Can a double digest be performed with PI-SceI and I-CeuI? A: Yes, since I-CeuI has 50% activity in PI-SceI buffer, both digestions can be done simultaneously in the unique PI-SceI buffer. Q: Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers? A: NEB is excited to announce that we have switched BSA-containing reaction buffers (NEBuffer 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We are also in the process of moving all restriction enzyme formulations to contain rAlbumin. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price.